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ORIGINAL ARTICLE
Year : 2012  |  Volume : 1  |  Issue : 1  |  Page : 6

Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant


1 Isfahan Water and Wastewater Company, Isfahan; Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Environment Research Center, Isfahan University of Medical Sciences (IUMS), Isfahan, Iran
3 Isfahan Water and Wastewater Company, Isfahan, Iran
4 Infectious Diseases and Tropical Medicine Research Center, IUMS, Isfahan, Iran
5 Science and Research Branch, Islamic Azad University, Tehran, Iran
6 Food Security Research Center, IUMS, Isfahan, Iran

Correspondence Address:
Parinaz Poursafa
Hezar Jerib Avenue, Isfahan University of Medical Sciences, Postal code: 81676 36954, Isfahan
Iran
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Source of Support: Funded by the Researches Council of Isfahan Water and Sewage Company, Research Project No. 88/17469., Conflict of Interest: None


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Aims: There is limited data on the occurrence of E. Coli O157: H7 in water. Therefore, this study aims to detect E. Coli O157: H7 in the Water Treatment Plant (WTP). Materials and Methods: This cross-sectional study was conducted in Isfahan WTP, central of Iran. Immunological methods were implemented with anti-serum kits and the molecular method of reverse transcription-polymerase chain reaction (RT-PCR) was used to detect E. Coli O157: H7 in eight locations of the WTP; the sludge of the sedimentation basin and filter backwash water were also monitored. The survival of E. Coli O157: H7 in the sludge samples of the sedimentation basin was indicated by the formation of agglutination particles using the immunological method, and through indicator probes using the RT-PCR method. Results: E. Coli O157: H7 was not detected in the water samples from the WTP units. The removal percent of total coliforms (TC), fecal coliforms (FC), and Heterotrophic Plate Count (HPC), respectively, were as follows: 59.5, 49, and 54.8% in the sedimentation basin; 66, 45.8, and 57% in the ozonation system; 98.8, 98, and 78.8% in the filtration system; and 96, 100, 91% in the disinfection system. Conclusions: This study revealed the existence of the pathogenic coliform of E. Coli O157: H7 in the sludge of the sedimentation basin. The absence of E. Coli O157: H7 in the finished water indicated that the WTP units were able to eliminate these pathogenic bacteria before reaching the final units of the plant, including the filtration and disinfection systems.


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