Print this page Email this page
Users Online: 219
Home About us Editorial board Search Browse articles Submit article Instructions Subscribe Contacts Login 
Year : 2012  |  Volume : 1  |  Issue : 1  |  Page : 6

Detection of E. coli O157: H7 by immunological and real-time PCR methods in the water treatment plant

1 Isfahan Water and Wastewater Company, Isfahan; Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Environment Research Center, Isfahan University of Medical Sciences (IUMS), Isfahan, Iran
3 Isfahan Water and Wastewater Company, Isfahan, Iran
4 Infectious Diseases and Tropical Medicine Research Center, IUMS, Isfahan, Iran
5 Science and Research Branch, Islamic Azad University, Tehran, Iran
6 Food Security Research Center, IUMS, Isfahan, Iran

Correspondence Address:
Parinaz Poursafa
Hezar Jerib Avenue, Isfahan University of Medical Sciences, Postal code: 81676 36954, Isfahan
Login to access the Email id

Source of Support: Funded by the Researches Council of Isfahan Water and Sewage Company, Research Project No. 88/17469., Conflict of Interest: None

Rights and PermissionsRights and Permissions

Aims: There is limited data on the occurrence of E. Coli O157: H7 in water. Therefore, this study aims to detect E. Coli O157: H7 in the Water Treatment Plant (WTP). Materials and Methods: This cross-sectional study was conducted in Isfahan WTP, central of Iran. Immunological methods were implemented with anti-serum kits and the molecular method of reverse transcription-polymerase chain reaction (RT-PCR) was used to detect E. Coli O157: H7 in eight locations of the WTP; the sludge of the sedimentation basin and filter backwash water were also monitored. The survival of E. Coli O157: H7 in the sludge samples of the sedimentation basin was indicated by the formation of agglutination particles using the immunological method, and through indicator probes using the RT-PCR method. Results: E. Coli O157: H7 was not detected in the water samples from the WTP units. The removal percent of total coliforms (TC), fecal coliforms (FC), and Heterotrophic Plate Count (HPC), respectively, were as follows: 59.5, 49, and 54.8% in the sedimentation basin; 66, 45.8, and 57% in the ozonation system; 98.8, 98, and 78.8% in the filtration system; and 96, 100, 91% in the disinfection system. Conclusions: This study revealed the existence of the pathogenic coliform of E. Coli O157: H7 in the sludge of the sedimentation basin. The absence of E. Coli O157: H7 in the finished water indicated that the WTP units were able to eliminate these pathogenic bacteria before reaching the final units of the plant, including the filtration and disinfection systems.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded470    
    Comments [Add]    

Recommend this journal