Evaluation of bio-aerosols concentration in the different wards of three educational hospitals in Iran
Heshmatollah Nourmoradi1, Mohammad Mehdi Amin2, Maryam Hatamzadeh2, Mahnaz Nikaeen2
1 Department of Environmental Health Engineering, School of Health, Ilam University of Medical Sciences, Ilam, Iran
2 Environment Research Center, Isfahan University of Medical Sciences (IUMS), Isfahan, Iran and Department of Environmental Health Engineering, School of Health, IUMS, Isfahan, Iran
|Date of Web Publication||31-Dec-2012|
Environment Research Center, Isfahan University of Medical Sciences, Isfahan
Source of Support: Isfahan University of Medical Sciences, Isfahan, Iran, Conflict of Interest: None
Aims: The aim of this study was to evaluate the bioaerosols level in the various parts of three educational hospitals of Isfahan, Iran.
Materials and Methods: The collection of bioaerosols (including bacterial and fungal microorganisms) was carried out with one-stage Anderson sampler. The sampling was carried out at the height of 1.5 m from the floor of various hospitals wards (infectious, surgery, urology wards, and operating room). The volume of each sample was determined based on pre-tests carried and was about 50 L. After sampling, the samples were incubated and analyzed. The effect of various environmental conditions including humidity, temperature, and outdoor bioaerosol levels was also investigated.
Results: The lowest numbers of fungal and bacterial concentration were obtained in operating rooms of the hospitals and the highest concentration was observed in infectious disease wards of hospital 1 and 2 and surgery ward of hospital 3. The bacterial concentration was observed to be higher in hospital wards than outdoor, except hospitals' operating rooms.
Conclusion: The findings showed that the bioaerosols level in the hospitals was relatively high. The higher levels of indoor bacteria than outdoor might be associated with the presence of patients, their activity, unsuitable ventilation, and disinfection. Therefore, environmental monitoring and control measures are required to improve hospital environmental quality especially in the wards with immune deficiency patients.
Keywords: Bacteria, bioaerosol, fungi, hospital
|How to cite this article:|
Nourmoradi H, Amin MM, Hatamzadeh M, Nikaeen M. Evaluation of bio-aerosols concentration in the different wards of three educational hospitals in Iran. Int J Env Health Eng 2012;1:47
|How to cite this URL:|
Nourmoradi H, Amin MM, Hatamzadeh M, Nikaeen M. Evaluation of bio-aerosols concentration in the different wards of three educational hospitals in Iran. Int J Env Health Eng [serial online] 2012 [cited 2019 Sep 16];1:47. Available from: http://www.ijehe.org/text.asp?2012/1/1/47/105346
| Introduction|| |
Bioaerosols are airborne particles which include living organisms such as bacteria, viruses, fungi, and their metabolites. ,, The size of bioaerosols ranges from 0.3 to 100 μm. Particles smaller than 10 μm have received much more attention from the health point of view.  Bioaerosols constitute 5-34% of the indoor air pollution.  Bioaerosols' adverse health effects include infectious and respiratory diseases, acute toxic effects, allergy, and cancer. , Bioaerosols enter the human body via several routes such as inhalation, ingestion, and skin contact. Inhalation is the most important route for transmission of bioaerosols into the body. Unlike chemicals, exposure to bioaerosols does not have any health threshold due to the type of microorganisms, entrance way, and difference in individual immune response.  Nowadays, hospitals and occupational infections are growing due to the exposure to bioaerosols. Therefore, it is necessary to investigate the bioaerosols, extensively.  The presence of bioaerosols in hospital wards is due to the presence of patients, their activities, inadequate ventilation, and incorrect disinfection.  Penicillium and Aspergillus species, Legionella pneumophila, Francisella tularensis, Bacillus anthracis, Yersinia More Details pestis, Trichoderma species, Mycobacterium tuberculosis, and Variola virus are the most important microorganisms which caused disease through the air.  Air-borne nosocomial infections are the main causes of pneumonia and surgical wound infection,  especially in immunocompromised patients.  Air conditioning and filtration equipment can be used to reduce the airborne fungi concentration.  Decrease in bacteria concentration in the air of the operating room also reduces the post-infection of surgery wounds. Bioaerosols monitoring in hospitals may provide useful information for epidemiological studies of nosocomial infections and control of bioaerosols. , Given the importance of monitoring of bioaerosols in health care settings, the aim of this study was to investigate the bioaerosols concentration in the air of various parts of three educational hospitals of Isfahan University of Medical Sciences, Iran.
| Materials and Methods|| |
This study was carried out on the microbial quality of air of three educational hospitals of Isfahan, Iran, in 2010. The operating room, surgery, urology, infectious, and special care wards of the hospitals were investigated. Also, outdoor sampling was done to find out the relationship between the bioaerosols concentration in the outdoor and indoor air. 130 samples of the air, including 5 times for any ward (65 fungal samples and 65 bacterial samples), were biweekly collected.
A one-stage Anderson sampler was used for bioaerosols sampling [Figure 1]. The sampler was located at a height of about 1.5 m from the floor (at the human breathing zone). Sampling was carried out for 5 min at the rate of 10 L/min (sample volume was determined based on pre-tests). After sampling, the samples were quickly transferred to the laboratory using a cold box and incubated. Temperature and humidity measurements were carried out using a portable instrument (KIMO, AMI 300) to determine the association between the bioaerosol concentrations and environmental conditions.
Sabouraud dextrose agar medium (Merck Co, Germany) containing chloramphenicol antibiotic was used for the determination of fungal bioaerosols. The fungal samples were incubated at ambient temperature (25°C) for a period of 5-7 days. The fungal colonies were counted and identified using a microscope (with a magnification of 40). Trypticase Soy Agar (TSA) containing nystatin (250 mg/L) was also provided from Merck Co. Germany and used for the sampling of bacteria. The concentration of bioaerosols has been reported as cfu/m 3 . The bacterial samples were transferred to the incubator (37°C) for a period of 24-48 h. Bacterial colonies were identified using the Gram staining and biochemical tests (catalase and coagulase).
| Results|| |
[Figure 2], [Figure 3], [Figure 4] show the mean number of fungi and bacteria in the various parts of the hospitals air. The lowest fungal and bacterial colonies in the air of three hospitals were found in the operating room.
|Figure 2: The number of fungi and bacteria in the air of hospital 1 wards|
Click here to view
|Figure 3: The number of fungi and bacteria in the air of hospital 2 wards|
Click here to view
|Figure 4: The number of fungi and bacteria in the air of hospital 3 wards|
Click here to view
The highest concentration of bacteria and fungi was detected in the infectious diseases ward of the hospitals 1 and 3 and surgery ward of hospital 2. The average number of fungal spores in the outdoor air of the hospitals was higher than the average number of fungi in the air of various parts of the hospitals. Whereas, the number of bacterial colonies in the outdoor air of the hospitals was lower than the air of various parts of the hospitals, except in the operating room. [Table 1] shows that the most common fungal species in the indoor air of the hospitals were Penicillium spp. and black fungi (mostly, Alternaria spp. and Cladosporium spp.), respectively. The most common fungi in the outdoor air were Penicillium spp. (40.6-60.2%) and black fungi (35.5-48.1%) similar to the hospital wards. Staphylococcus aureus (21.71%), negative-coagulase Staphylococcus species (77.3%), and Gram-positive bacilli with side spores (0.99%) constituted the bacterial bioaerosols in the air of various parts of hospital 1. The S. aureus and negative-coagulase Staphylococcus species in hospital 2 were 22.73% and 77.27%, respectively. The S. aureus, coagulase-negative Staphylococcus, and Streptococcus species in the air samples of the hospital 3 were 20.85%, 78.2%, and 0.95%, respectively.
|Table 1: Frequency of predominant fungi in the air of various wards of the hospitals|
Click here to view
| Discussion|| |
Bioaerosols, smaller than 10 μm, are of greatest concern for human health. Exposure to bioaerosols in hospitals may result in infection, especially in immunocompromised patients. [Figure 2], [Figure 3], [Figure 4] show the average number of fungi and bacteria in the air of various parts of the hospitals. The results show that the lowest fungal and bacterial contamination was found in the operating rooms. The number of bioaerosols in the operating room of the hospitals showed that hospital 1 has a better quality (P < 0.05). Azizifar et al. reported that the lowest fungi level was found in the operating room (94 cfu/m 3 ) of Qom Kamkar hospital, Iran.  Perdelli et al. also reported that the lowest amount of fungal contamination was in the operating room (operating theater) of hospital air (12±14 cfu/m 3 ).  With regard to sterilization process of operating rooms, bioaerosols concentration should be very low. The presence of bioaerosols in the operating room may be due to the inefficiency of ventilation and sterilization systems. The results of Favero et al. showed that the emission of microbial contamination from skin, hair, and respiratory tract of the person is one of the important causes for the presence of bioaerosols in the air of the operating rooms.  The highest load of bacterial contamination in hospital 1 was detected in the infectious ward which was equal to 448 cfu/m 3 . High concentration of bacteria in the infectious ward may be due to the presence of patient with infectious diseases and inadequate ventilation in this section. Azizfar et al. have also reported the highest fungal contamination in the infectious ward of the Qom Kamkar hospital (300 cfu/ m 3 ).  ANOVA statistical analysis results do not approve significant difference between the mean total number of bacteria and fungi in the air of hospitals 1, 2, and 3 (P > 0.05). The statistical test results also showed a significant difference between the concentrations of fungi and bacteria in the air of various parts of hospital 1, except the urology and surgery wards (P < 0.05). There is also a statistical difference between the average number of fungi and bacteria in the operating room and other wards of hospitals 2 and 3 (P < 0.05). Statistical analysis also approves the high concentration of bacteria in the air of wards compared to outdoor air, except the operating room (P < 0.01). This may be due to the presence of patients, their activities, inadequate ventilation, and unsuitable disinfection of the hospitals.  But the average number of fungi in the outdoor air is higher than various parts of the hospital. The presence of fungi spores in the indoor air may be due to contact with the outdoor air. However, the number of fungi must be less than 15 cfu/m 3 in the hospitals equipped with a filtration system.  Air humidity and temperature were measured to evaluate the effect of environmental factors on bioaerosols concentration. Pearson's correlation analysis showed that the environmental conditions (humidity and temperature) have no significant effects on the bioaerosols levels (P > 0.05). Obbard et al. found a significant relationship between the concentration of bacteria, population density, and air humidity. They also realized that there is a relationship between the outdoor and indoor bacteria concentration.  However, in the current study, statistical analysis did not confirm a correlation between the outdoor and indoor bioaerosols concentration (P > 0.05). [Table 1] shows the frequency of dominant fungal species separated from the hospitals' air. The results show that the most common fungi separated from the hospitals' air were Penicillium and black fungi. Aspergillus species accounted 1.79-2.37% of fungal bioaerosols in the current study. Li et al. reported that the most common fungal species in the operating rooms of Taiwan hospital as Penicillium spp.  Ekhaise et al. also found that Penicillium spp. and Aspergillus spp. were the most fungal species in the air of Government Owned hospitals in Benin City, Nigeria.  Mahdavi et al. reported that the most common fungus in the air of Babol hospital (Iran) was Penicillium spp.  Hashemi et al. also found that the most common fungal species were Cladosporium and Penicillium in the Shariati hospital of Tehran, Iran.  Analysis of the bacterial aerosols in the Shariati hospitals showed that the most common bacterial species were negative-coagulase Staphylococcus species (77.27-78.2%) and S. aureus (20.85-21.71%). Ekhaise et al. reported that the average number of bacteria in the air of a hospital in Nigeria was 15-52 cfu/m 3 and Staphylococcus was the most common bacteria.  According to a study by Pastuszka et al. on the air of a hospital in Poland, the average number of bacteria was reported in the range of 100-1000 cfu/m 3 and the most common bacterial species were Staphylococcus and Micrococcus (57-78%). 
| Conclusion|| |
According to the results obtained in the current study, the bioaerosols concentration in the above hospitals is relatively high. The higher number of bacteria in the hospitals than outdoor air can be attributed to the patients, their activities, inadequate ventilation, and incorrect disinfection. Therefore, some measures including installation of filter in the inlet air to the hospital wards, avoiding opening windows for natural ventilation and regular disinfection of the hospital, are suggested to reduce the concentration of bioaerosols and improve the air quality. Because of the presence of bioaerosols in the operating room, it is also recommended that the air filters and sterilization system (UV lamps) be under constant control to ensure their proper operation.
| Acknowledgments|| |
The authors wish to thank the vice-chancellery of research, Isfahan University of Medical Sciences (IUMS), for financial support of this project (#288284). The authors also thank the Environmental Health authorities and the head nurses of various parts of IUMS hospitals for their kind cooperation.
| References|| |
|1.||Ruzer LS, Harley NH. Aerosols handbook: Measurement, dosimetry, and health effects. New York. CRC Press; 2004. |
|2.||Douwes J, Thorne P, Pearce N, Heederik D. Bio-aerosol health effects and exposure assessment: Progress and prospects. Ann Occup Hyg 2003;47:187-200. |
|3.||Stetzenbach LD, Buttner MP, Cruz P. Detection and enumeration of airborne biocontaminants. Curr Opin Biotechnol 2004;15:170-4. |
|4.||Cox CS, Wathes CM. Bio-aerosols in the environment. In: Cox CS, Wathes CM, editors. Bio-aerosols Handbook. Boca Raton, FL: Lewis Publishers; 1995;11-4. |
|5.||Srikanth P, Sudharsanam S, Steinberg R. Bio-aerosols in indoor environment: Composition, health effects and analusis. Indian J Med Microbiol 2008;26:302-12. |
|6.||Macher J, Ammann HA, Burge HA, Milton DK, Morey PR, editors. Chapter 1. In: Bio-aerosols: Assessment and Control. Cincinnati: ACGIH; 1999;1-5. |
|7.||Beggs CB. The airborne transmission of infection in hospital buildings: Fact or fiction? Indoor Built Environ 2003;12:9-18. |
|8.||Ayliffe G, Babb JR, Taylor LJ. Infection and the spread of microorganisms, Chapter 3. In: Hospital Acquired Infections: Principles and prevention, 3 rd ed. Oxford: Butterworth Heinemann publications; 1999;8-40. |
|9.||Kowalski WJ. The epidemiology and aerobiological pathways of airborne nosocomial infections and methods of air and surface disinfection. HPAC Engineering: Air-Treatment Systems for Controlling Hospital-Acquired Infections 2007. Available from: http://www.hpac.com/Issue/Article/44503/AirTreatment_Systems_for_Controlling_HospitalAcquired_Infections. [Last accessed on 2007 Aug 03]. |
|10.||Hajjeh RA, Warnock DW. Counterpoint: Invasive aspergillosis and the environment-rethinking our approach to prevention. Clin Infect Dis 2001;33:1549-52. |
|11.||Curtis L, Ross M, Persky V, Scheff P, Wadden R, RamakrisnanV, Hryhorczuk D. Bioaerosol concentrations in the quad cities 1 year after the 1993 Mississippi river floods. Indoor Built Environ 2000;9:35-43. |
|12.||Lidwell OM. Airborne bacteria and surgical infection. Am J Med 1981;70:693-7. |
|13.||Azizifar M, Jabbari H, Naddafi K, Nabizadeh R, Tabraei Y, Solgi A. A survey on qualitative and quantitative fungal contamination of different air wards of Qom (Kamkar) city hospital in 2007. Qom Univ Med Sci J 2009;3:25-30. |
|14.||Perdelli F, Cristina ML, Sartini M, Spagnolo AM. Fungal contamination in hospital environments. ICHE 2007;27:44-7. |
|15.||Favero MS, Puleo JR, Marshall JH, Oxborrow GS. Comparison of microbial contamination levels among hospital operating rooms and industrial clean rooms. Appl Microbiol 1968;16:480-6. |
|16.||Streifel AJ. Air cultures for fungi. In: Gilcrist M, editor. Clinical Microbiology Procedures Handbook. Washington, DC: American Society for Microbiology Press; 1992. |
|17.||Obbard JP, Fang LS. Airborne concentrations of bacteria in a hospital environment in Singapore. Water Air Soil Pollut 2003;144:333-41. |
|18.||Li CS, Hou PA. Bioaerosol characteristics in hospital clean rooms. Sci Total Environ 2003;305:169-76. |
|19.||Ekhaise FO, Ighosewe OU, Ajakpovi OD. Hospital indoor airborne microflora in private and government owned hospitals in Benin city, Nigeria. World J of Med Sci 2008;3:19-23. |
|20.||Mahdavi Omran S, Sheidfar M. A survey of the mycological flour contamination in Babol hospitals. Med J Tabriz Univ Med Sci 2000;48:45-52. |
|21.||Hashemi J, Sharhani M. A survey comparative saprophytes fungal existent indoor and equipments research center for blood and incology and clinical patients examples for trans-plant patient in Shryati hospital in Tehran. Tehran Univ Med J 2002;62:175-9. |
|22.||Pastuszka JS, Marchwiska WE, Wlazo A. Bacterial aerosol in Silesian hospitals: Preliminary results. Pol J of Environ Stud 2005;14:883-90. |
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
|This article has been cited by|
||Assessment of Airborne Bacterial and Fungal Communities in Selected Areas of Teaching Hospital, Kandy, Sri Lanka
| ||Premina Sivagnanasundaram,R. W. K. Amarasekara,R. M. D. Madegedara,Anuradha Ekanayake,D. N. Magana-Arachchi |
| ||BioMed Research International. 2019; 2019: 1 |
|[Pubmed] | [DOI]|
||Concentration and type of bioaerosols before and after conventional disinfection and sterilization procedures inside hospital operating rooms
| ||Mansooreh Dehghani,Armin Sorooshian,Shahrokh Nazmara,Abbas Norouzian Baghani,Mahdieh Delikhoon |
| ||Ecotoxicology and Environmental Safety. 2018; 164: 277 |
|[Pubmed] | [DOI]|
||Types and Levels of Bioaerosols in Healthcare and Community Indoor Settings in Iran
| ||Abdolmajid Ghasemian,Sepideh Khodaparast,Fatemeh Savaheli Moghadam,Farshad Nojoomi,Hasan Rajabi Vardanjani |
| ||Avicenna Journal of Clinical Microbiology and Infection. 2016; In Press(In Press) |
|[Pubmed] | [DOI]|